Wild kind of attB and you will attP sequences aimed showing new respective core binding and spacer sequences

Wild kind of attB and you will attP sequences aimed showing new respective core binding and spacer sequences Lanes: M1, 1 kb DNA hierarchy; HT, genomic DNA out of HT1080; step 3, 11, 18*, 19 and 21, genomic DNA out-of focused clones; pP4X (ten eight , 10 8 , 10 nine ), duplicates off linearized[…]